描述
Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.
Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension
规格
Concentration (molarity): 10 mM
Label or Dye: Aminoallyl
Labeling Method: Direct Labeling
Product Size: 0.1 mL
